RES eDNA and Innovation Leadership program

4 min
RES eDNA and Innovation Leadership program
1:37

The Mule Train Stream and Wetland Mitigation Bank near Nashville restores nearly four miles of streams using environmental DNA (eDNA) to detect aquatic species. This innovative tool simplifies habitat surveys, supporting conservation and advancing mitigation efforts.

At the Mule Train Stream and Wetland Mitigation Bank in Sumner County, just north of Nashville, a groundbreaking approach is being employed to enhance restoration efforts. The primary focus at this site is the restoration of nearly four miles (16,000–20,000 feet) of streams. To support this work, the team is utilizing environmental DNA (eDNA) technology, a cutting-edge tool that provides a deeper understanding of aquatic habitats.

Traditional survey methods often require labor-intensive processes, like flipping rocks to locate species of interest, and may miss elusive or rare organisms. eDNA offers a more efficient alternative by analyzing water samples to detect genetic material shed by organisms in the environment. This allows researchers to determine the presence or absence of target species, even in areas where physical evidence is hard to find.

eDNA sampling has been particularly valuable in identifying the distribution of aquatic species across the watershed. By comparing the collected DNA to published databases, the team can confirm whether a species is present in the main stem or upstream tributaries without extensive manual surveys. This not only improves efficiency but also provides a broader ecological picture to inform restoration strategies.

The use of eDNA at Mule Train is a testament to innovation in the field of ecological restoration. The team is training in this technology and exploring its applications for regulators and clients, making it a promising tool for advancing mitigation bank practices.